Introduction
Although the name is a misnomer, Western blotting is an assay to identify a protein and it's weight. It takes advantage of antibodies to detect a specific protein in a given sample. the antibody itself could be detected with auto radiography or an enzyme driven light producing compounds.
Important material used in this assay

1. A protein sample (to protein content be identified)
2. A detergent, like SDS, to denature the proteins in sample
3. GEL Electrophores
4. Nitrocellulose paper or PVDF (Polyvinylidene fluoride)
5. Antibody

Critical Steps

1. Proteins are denatured with SDS
2. Proteins are separated with EF, according to their weight
3. In a so called transfer procedure, the proteins are transfered to a nitrocellulose paper
4. Blocking the remaining space with an inert proein like casein or BSA
5. Incubation of the paper with antibody
6. A brief washing
7. Visualization of Antibody

Applications
This assay is mainly used for identification of a specific protein and its properties(Weight) in a sample.

• Molecular weight estimation.
• Identification of specific protein in sample.
• Quantification of protein.